时间和剂量依赖性的细胞毒性ioxitalamate indigocarmine人类髓核细胞。

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金KH、金y栏苏,公园,公园司法院,下巴DK, HS金KS,曹

时间和剂量依赖性的细胞毒性ioxitalamate indigocarmine人类髓核细胞。

脊柱j . 2013; 13 (5): 564 - 71。doi: 10.1016 / j.spinee.2013.01.019。Epub 2013年2月11日。

PubMed ID

23406968 (在PubMed

]

文摘

背景背景:Ioxitalamate离子碘化造影剂(Telebrix 300)是一种常用的内容或经皮内窥镜椎间盘摘除(PELD),尽管它有副作用,如过敏性休克和肾毒性。Indigocarmine是一种有机化合物和一种独特的蓝色染料,常用在PELD酸性染色,退化髓核(NP)。尽管ioxitalamate和indigocarmine广泛应用于脊柱手术,没有报告对NP细胞的影响。我们研究了ioxitalamate和indigocarmine NP细胞的毒性。目的:确定ioxitalamate和indigocarmine NP细胞毒性的体外。研究设计:体外毒性的对照研究ioxitalamate和indigocarmine人类NP细胞。方法:通过椎间盘髓核细胞获得从腰椎盘病人和孤立。髓核细胞培养在三维(3 d)海藻酸珠子用0.001,0.1,10和100毫克/毫升ioxitalamate, 0.00001, 0.001, 0.1,和10毫克/毫升indigocarmine,或者两者的混合1、2或3天。用台盼蓝染色法分析了活细胞。使用膜联蛋白V和荧光激活细胞分类分析propidium碘化海藻酸和3 d珠疣状进行识别生活,凋亡、坏死细胞。 RESULTS: Ioxitalamate, indigocarmine, and their combination induced statistically significant NP cell injury that was both time- and dose dependent (p<.05). Also, at the same concentration, ioxitalamate was more cytotoxic than was indigocarmine or the combination (p<.05). All three treatments also showed dose-dependent cytotoxicity according to flow cytometry and immunostaining. CONCLUSIONS: Ioxitalamate and indigocarmine are toxic to human NP cells in vitro in a time- and dose-dependent manner. We assume that ioxitalamate and indigocarmine may have similar effects in patients undergoing discography and PELD. Thus, we suggest that ioxitalamate and indigocarmine should be used carefully at low concentrations.

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药物

Ioxitalamic酸