金属结合蛋白的识别在人类肝癌行通过固定化金属亲和色谱法和质谱法。
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她YM, Narindrasorasak年代,杨年代,Spitale N,罗伯茨EA, Sarkar B
金属结合蛋白的识别在人类肝癌行通过固定化金属亲和色谱法和质谱法。
摩尔细胞蛋白质组学。2003;12月2 (12):1306 - 18。Epub 2003 10月7。
- PubMed ID
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14534351 (在PubMed]
- 文摘
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metalloproteome被定义为一组的蛋白质metal-binding能力被金属离子或metal-binding网站。为每个金属不同metalloproteome可能存在。质谱表征metalloproteomes提供有价值的信息有关细胞的金属生理和性格metal-associated疾病。我们调查了三个人类肝癌行:铜和锌metalloproteomes消息灵通的G2 Mz-Hep-1,保留许多正常的人类肝细胞的功能特点,SK-Hep-1,分化不良。另外我们研究一个正常的人类肝脏标本和消息灵通的G2细胞耗尽体外细胞铜。我们使用matrix-assisted激光解吸电离和电喷雾四极杆飞行时间质谱分析获得的肽序列的胰蛋白酶的消化消化凝固态的金属结合蛋白或多肽的固定化金属亲和层析柱含有铜或锌。主要是高蛋白质丰度确定。Cu-binding蛋白质识别包括烯醇酶、白蛋白、转铁蛋白和乙醇脱氢酶以及某些细胞内伴护蛋白质。的铜锌metalloproteome metalloproteome并不相同。肽结合实验表明,铜协调喜欢残留的顺序组氨酸>蛋氨酸>半胱氨酸。 Although the Cu metalloproteome was similar from line to line, subtle differences were apparent. Gel profiling showed more extensive variation in expression of annexin II in SK-Hep-1 and Mz-Hep-1 than in Hep G2 and normal liver tissue. Glycerylphosphorylethanolamine was identified as a post-translational modification at residue Glu-301 of elongation factor 1-alpha in Hep G2. Intracellular copper depletion was associated with loss of the glycerylphosphoryl side group. These findings suggest that post-translational modification could be affected by intracellular actions of copper. Comparison of the Cu and Zn metalloproteomes in Hep G2 with a published general proteome of Hep G2 disclosed little overlap (Seow, T. K., et al. (2001) Proteomics 1, 1249-1263). Proteins in the metalloproteomes of human hepatocytes can be identified by these methods. Variations in these metalloproteomes may have important physiological relevance.
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药物 航空公司 类 生物 药理作用 行动 铜 血清白蛋白 蛋白质 人类 没有粘结剂细节