净化和马睾丸细胞色素p - 450芳香化酶的特征:与人类的酶。

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Moslemi年代,Vibet,帕帕多普洛斯V, Camoin L, Silberzahn P,盖拉德杰

净化和马睾丸细胞色素p - 450芳香化酶的特征:与人类的酶。

比较生物化学杂志B生物化学杂志。1997年9月,118(1):217 - 27所示。

PubMed ID

9418012 (在PubMed

]

文摘

细胞色素p - 450芳香化酶是由五个色谱纯化步骤从成年公马睾丸。首次分离NADPH-cytochrome p - 450还原酶(还原酶)omega-aminohexyl-Sepharose 4 b提纯上同质性伴刀豆球蛋白A-Sepharose 4 b, hydroxyapatite-Sepharose 4 b, DEAE-Sepharose CL-6B和第二个hydroxyapatite-Sepharose 4 b。另一方面,马睾丸的方法进行了净化和鼠肝脏还原酶,使芳香化酶活性在体外的重构,实现了每个物种在一个色谱一步一个腺苷2 ',5 ' -diphosphate-agarose亲和力列。分析在SDS /页面显示有明显的单人乐队的分子质量53岁,82年,和80 kDa纯化马睾丸细胞色素p - 450芳香化酶(eAROM),马睾丸分别还原酶和鼠肝脏还原酶。eAROM显示一个稳定的时间和浓度活动至少2个月当储存在-78度c,它是一个高度疏水蛋白质由505残留物和直接测序的氨基端部分显示良好的同源性与人类相比芳香化酶。当deglycosylated N-glycosidase-F eAROM明显分子质量从53个减少到51 kDa电泳显示,其活动,然而,没有受损。eAROM展品更高的亲和力比19-norandrogens雄激素,大约3公里的值,16和170 nM雄烯二酮(A)、睾酮(T)和19-nortestosterone (19-NT)分别。然而,它使芳香19-norandrostenedione (19-NA)更有效地比雌酮(E1)形成为4.27 vs 3.54 pmol最低为1分别micrograms-1 (P < 0.01)。eAROM孵化后与放射性标记和类固醇对高效液相色谱分离,E1, 19-hydroxyandrostenedione (19-OHA)和19-oxoandrostenedione (19-oxoA)积累在孵化中时间的方式。4-hydroxyandrostenedione (4-OHA),一个自杀的芳香化酶抑制剂,导致时间失活的酶。 Whereas the activity of eAROM was unchanged in the presence of K+ (up to 250 mM), it was increased in the presence of EDTA (up to 50 mM) and decreased in the presence of DTT or Mg2+ (from 25 mM). We conclude that: (a) eAROM is a glycoprotein, however, deglycosylation by N-glycosidase-F does not appear to impair its activity, (b) eAROM aromatizes really both androgens and 19-norandrogens having a higher affinity for androgens, (c) the intermediary compounds of aromatization 19-OHA and 19-oxoA appear to be synthesized by the same active site that synthesizes E1 as the final product, (d) the inhibition of eAROM by increasing concentrations of Mg2+ and the stimulation of its activity by EDTA, taken together, indicate the importance of negatively charged residues in the polypeptide chain of equine aromatase, which play a role in enzymatic activity.

DrugBank数据引用了这篇文章

药物酶

药物	酶	类	生物	药理作用	行动
Edetate钙二钠无水	细胞色素P450 19 a1	蛋白质	人类	未知的	调制器	细节
Edetate二钠无水	细胞色素P450 19 a1	蛋白质	人类	未知的	调制器	细节
依他酸	细胞色素P450 19 a1	蛋白质	人类	未知的	底物	细节