Deoxyribodipyrimidine photo-lyase

Details

Name
Deoxyribodipyrimidine photo-lyase
Synonyms
  • 4.1.99.3
  • DNA photolyase
  • Photoreactivating enzyme
  • phrA
Gene Name
phr
Organism
Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1)
Amino acid sequence
>lcl|BSEQ0016945|Deoxyribodipyrimidine photo-lyase MAAPILFWHRRDLRLSDNIGLAAARAQSAQLIGLFCLDPQILQSADMAPARVAYLQGCLQ ELQQRYQQAGSRLLLLQGDPQHLIPQLAQQLQAEAVYWNQDIEPYGRDRDGQVAAALKTA GIRAVQLWDQLLHSPDQILSGSGNPYSVYGPFWKNWQAQPKPTPVATPTELVDLSPEQLT AIAPLLLSELPTLKQLGFDWDGGFPVEPGETAAIARLQEFCDRAIADYDPQRNFPAEAGT SGLSPALKFGAIGIRQAWRAASAAHALSRSDEARNSIRVWQQELAWREFYQHALYHFPSL ADGPYRSLWQQFPWENREALFTAWTQAQTGYPIVDAAMRQLTETGWMHNRCWMIVASFLT KDLIIDWRRGEQFFMQHLVDGDLAANNGGWQWSASSGMDPKPLRIFNPASQAKKFDATAT YIKRWLPELRHVHPKDLISGEITPIGRRGYPAPIVNHNLRQKQFKALYNQLKAAIAEPEA EPDS
Number of residues
484
分子量
54461.235
Theoretical pI
6.74
GO Classification
Functions
deoxyribodipyrimidine photo-lyase activity/DNA binding/nucleotide binding
Processes
DNA repair/protein-chromophore linkage
General Function
Nucleotide binding
Specific Function
Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ultraviolet radiation.
Pfam Domain Function
Transmembrane Regions
Not Available
Cellular Location
Not Available
Gene sequence
>拼箱| BSEQ0016946 | Deoxyribodipyrimidine photo-lyase(phr) GTGGCGGCTCCGATTCTGTTTTGGCACCGGCGTGACCTGCGCCTCAGCGACAACATTGGT CTGGCGGCGGCGCGGGCACAATCAGCGCAGCTGATCGGTCTGTTTTGCCTCGACCCTCAG ATTCTCCAGAGTGCTGACATGGCGCCAGCACGGGTTGCCTACCTGCAGGGCTGCCTACAG GAACTCCAGCAGCGTTATCAACAGGCGGGCAGTCGGCTGCTATTGCTCCAAGGGGATCCA CAACACCTGATCCCGCAACTGGCGCAGCAGCTTCAGGCGGAAGCAGTTTATTGGAACCAA GACATTGAACCCTACGGCCGCGATCGCGATGGGCAGGTCGCAGCCGCTCTGAAAACGGCA GGCATTCGGGCTGTTCAACTCTGGGATCAACTGCTCCATAGCCCCGATCAAATCCTCAGC GGCAGCGGTAATCCCTACAGCGTCTACGGGCCGTTTTGGAAAAATTGGCAGGCTCAGCCC AAGCCCACGCCGGTTGCCACACCGACAGAACTGGTCGATCTCTCACCAGAGCAACTAACT GCGATCGCACCTCTGCTGTTGTCGGAACTACCGACCCTGAAGCAGCTTGGCTTTGACTGG GACGGGGGTTTTCCAGTGGAGCCGGGGGAAACAGCGGCGATCGCCCGTCTTCAAGAATTT TGCGATCGCGCGATCGCCGACTACGATCCCCAGCGCAATTTTCCGGCGGAAGCAGGAACC TCGGGCTTGAGTCCCGCTTTGAAATTCGGGGCGATCGGTATTCGTCAGGCTTGGCGGGCT GCCAGTGCAGCCCATGCCCTCAGCCGCAGCGACGAAGCCCGCAACAGCATTCGCGTTTGG CAGCAGGAACTCGCTTGGCGGGAGTTCTATCAGCATGCGCTCTACCATTTCCCGAGCCTG GCCGATGGTCCCTATCGCTCGCTCTGGCAGCAATTCCCTTGGGAAAATCGCGAGGCTCTG TTCACGGCTTGGACCCAGGCGCAAACGGGCTATCCGATTGTTGATGCAGCGATGCGCCAA CTGACTGAAACCGGCTGGATGCACAACCGTTGCTGGATGATTGTTGCCAGCTTTTTGACC AAGGATTTGATCATCGACTGGCGGCGTGGGGAGCAGTTCTTTATGCAGCACTTGGTCGAT GGCGATCTGGCTGCAAATAACGGCGGTTGGCAGTGGAGCGCTTCCAGTGGCATGGATCCC AAACCGCTGCGGATTTTCAATCCTGCTAGTCAGGCCAAGAAGTTTGATGCCACGGCGACC TACATCAAACGTTGGCTGCCAGAACTGCGCCATGTCCATCCCAAGGACTTGATCAGTGGG GAGATCACGCCGATTGGACGCCGAGGCTATCCTGCCCCGATCGTGAATCACAACCTGCGC CAGAAACAGTTCAAGGCGCTCTACAACCAGCTCAAAGCGGCTATCGCTGAACCTGAGGCT GAGCCCGATTCATAG
Chromosome Location
Not Available
Locus
Not Available
External Identifiers
Resource Link
UniProtKB ID P05327
UniProtKB Entry Name PHR_SYNP6
GenBank Gene ID X07230
General References
  1. Yasui A, Takao M, Oikawa A, Kiener A, Walsh CT, Eker AP: Cloning and characterization of a photolyase gene from the cyanobacterium Anacystis nidulans. Nucleic Acids Res. 1988 May 25;16(10):4447-63. [Article]
  2. Sugita C, Ogata K, Shikata M, Jikuya H, Takano J, Furumichi M, Kanehisa M, Omata T, Sugiura M, Sugita M: Complete nucleotide sequence of the freshwater unicellular cyanobacterium Synechococcus elongatus PCC 6301 chromosome: gene content and organization. Photosynth Res. 2007 Jul-Sep;93(1-3):55-67. Epub 2007 Jan 9. [Article]
  3. Eker AP, Kooiman P, Hessels JK, Yasui A: DNA photoreactivating enzyme from the cyanobacterium Anacystis nidulans. J Biol Chem. 1990 May 15;265(14):8009-15. [Article]
  4. Weber S: Light-driven enzymatic catalysis of DNA repair: a review of recent biophysical studies on photolyase. Biochim Biophys Acta. 2005 Feb 25;1707(1):1-23. [Article]
  5. Tamada T, Kitadokoro K, Higuchi Y, Inaka K, Yasui A, de Ruiter PE, Eker AP, Miki K: Crystal structure of DNA photolyase from Anacystis nidulans. Nat Struct Biol. 1997 Nov;4(11):887-91. [Article]
  6. Kort R, Komori H, Adachi S, Miki K, Eker A: DNA apophotolyase from Anacystis nidulans: 1.8 A structure, 8-HDF reconstitution and X-ray-induced FAD reduction. Acta Crystallogr D Biol Crystallogr. 2004 Jul;60(Pt 7):1205-13. Epub 2004 Jun 22. [Article]
  7. Mees A, Klar T, Gnau P, Hennecke U, Eker AP, Carell T, Essen LO: Crystal structure of a photolyase bound to a CPD-like DNA lesion after in situ repair. Science. 2004 Dec 3;306(5702):1789-93. [Article]

Drug Relations

Drug Relations
DrugBank ID Name Drug group Pharmacological action? Actions Details
DB03147 Flavin adenine dinucleotide approved unknown Details