描述MOAT-C MOAT-D,新成员的MRP / cMOAT亚科的转运蛋白。
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Belinsky毫克,贝恩LJ, Balsara BB,甲壳JR Kruh GD
描述MOAT-C MOAT-D,新成员的MRP / cMOAT亚科的转运蛋白。
中华肿瘤杂志,1998年11月18日,90 (22):1735 - 41。
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9827529 (在PubMed]
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背景:多resistance-associated蛋白(MRP)和微管的multispecific有机阴离子转运蛋白(cMOAT)有机阴离子转运蛋白泵在细胞膜与细胞毒性药物的耐药性。我们之前已经确定MOAT-B MRP / cMOAT-related运输车,利用聚合酶链反应的方法。然而,表达序列标签(EST)数据库的分析表明,可能有额外的MRP / cMOAT-related转运蛋白。进一步定义MRP / cMOAT转运蛋白的亚科,我们使用EST探针分离为两个相关转运体蛋白质互补dna, MOAT-C MOAT-D。方法:MOAT-C和MOAT-D表达模式在人体组织是由RNA污点分析和基因的染色体定位是由荧光原位杂交。结果:MOAT-C预测编码1437 -氨基酸蛋白,在真核转运蛋白,是最密切相关的MRP, cMOAT, MOAT-B身份(大约36%)。然而,MOAT-C MRP和cMOAT不如MRP和cMOAT彼此身份(大约48%)。像MOAT-B MOAT-C缺乏一个氨基端membrane-spanning域,表明这种蛋白质的拓扑是同样与MRP和cMOAT截然不同。MOAT-D预测编码一个1527 -氨基酸蛋白是最接近已知的MRP(大约58%的身份)。MOAT-D cMOAT还高度相关(大约47%的身份)。 The presence of an N-terminal membrane-spanning domain indicates that the topology of MOAT-D is quite similar to that of MRP and cMOAT. MOAT-C transcripts are widely expressed in human tissues; however, MOAT-D transcript expression is more restricted. The MOAT-C and MOAT-D genes are located at chromosomes 3q27 and 17q21.3, respectively. CONCLUSIONS: On the basis of amino acid identity and protein topology, the MRP/cMOAT transporter subfamily falls into two groups; the first group consists of MRP, cMOAT, and MOAT-D, and the second group consists of MOAT-B and MOAT-C.