5的异质性上皮钠通道alpha-subunit信使rna蛋白质会导致不同的NH2-terminal变体。
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托马斯•CP Auerbach年代,斯托克斯JB,沃尔克KA
5的异质性上皮钠通道alpha-subunit信使rna蛋白质会导致不同的NH2-terminal变体。
杂志。1998;274 (5 1 Pt): C1312-23。
- PubMed ID
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9612219 (在PubMed]
- 文摘
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amiloride-sensitive上皮钠通道(钠)是由三个子单元:α,β和γ。人类alpha-ENaC亚基表达为至少两个成绩单(n . Voilley e . Lingueglia g . Champigny m·g·加尼姆r . Waldmann m . Lazdunski和p . Barbry。Proc。国家的。学会科学。美国91:247 - 251,1994)。确定这些成绩单的起源,我们alpha-ENaC基因的5 '末端。四个不同记录在他们的第一个外显子。外显子1拼接外显子2形成5 ' alpha-ENaC1结束,而外显子1 b分别出现并持续到外显子2 alpha-ENaC2形式。其他变体mrna, alpha-ENaC3 alpha-ENaC4,形成通过激活5 '外显子1 b内接头地点。尽管alpha-ENaC3和4并没有改变alpha-ENaC的开放阅读框,alpha-ENaC2包含上游atg, 59氨基酸添加到先前(alpha-ENaC1)的蛋白质。 To address the significance of these isoforms, both proteins were expressed in Xenopus oocytes. The cRNA for each alpha-ENaC transcript when combined with beta- and gamma-ENaC cRNA reconstituted a low-conductance ion channel with amiloride-sensitive currents of similar characteristics. We have thus identified variant alpha-ENaC mRNAs that lead to functional ENaC peptides.