体外研究的玫瑰红染色和毒性培养的牛角膜内皮细胞。
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李YC,公园CK,金女士,金正日JH
体外研究的玫瑰红染色和毒性培养的牛角膜内皮细胞。
角膜。1996年7月,15 (4):376 - 85。
- PubMed ID
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8776564 (在PubMed]
- 文摘
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它最近被报道,玫瑰红(RB)不是一个重要的染料,它有一个内在的毒性作用。因此,我们进行了一项研究来确定它的染色能力和内在的存在毒性。我们还研究了其对培养的牛角膜内皮细胞的增殖的影响。细胞染色尽管短曝光(1)光学荧光素显微镜下玫瑰红。细胞核染色第一,紧随其后的是细胞质;强度直接依赖于曝光时间。虽然整个细胞层没有污点的RB浓度0.001%,上述细胞层彩色广泛浓度为0.01%。5%浓度、细胞分离和溶解被指出。细胞变化的程度直接与RB的浓度和暴露时间成正比。细胞损伤包括细胞肿胀,胞浆内空泡形成、细胞分离、细胞溶解。 The presence of light augmented the toxic effect of RB on cultured endothelial cells and was statistically more significant than RB's intrinsic toxic effect. Exposure to RB concentrations > 0.01% induced statistically significant concentration-dependent inhibition of cell proliferation. These results suggest that rose bengal may not actually be a vital dye on cultured bovine corneal endothelial cells and that it has intrinsic inhibitory and toxic effects with additional phototoxic effects on corneal endothelial cells. Furthermore, cell proliferation was markedly inhibited after exposure to RB.