p12的功能角色,第四单元的人类DNA聚合酶三角洲。
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李H,谢B,周Y, Rahmeh, Trusa年代,张年代,高Y,李,李我的
p12的功能角色,第四单元的人类DNA聚合酶三角洲。
J生物化学杂志。2006年5月26日,281 (21):14748 - 55。Epub 2006年2月28日。
- PubMed ID
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16510448 (在PubMed]
- 文摘
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哺乳动物的DNA聚合酶δ(polδ)染色体DNA复制的关键酶,由四个单元如下:催化亚基;p125,紧密相关的p50单元;2、增殖细胞核抗原(PCNA)结合蛋白;和第四个亚基,p12。在这项研究中,的功能角色p12波尔三角洲亚基进行了研究。p12的inter-subunit交互单元被下拉由酵母2台混合动力分析和化验。这些化验显示,p12与p125 p50。这种双重互动p12表明它可能有助于稳定p125-p50交互。p12被证实是一个小说PCNA-binding蛋白质。这证实了识别PCNA-binding主题的N末端绑定化验和定点诱变。 The activities and reaction products of recombinant pol delta containing a p12 mutant defective in PCNA binding, as well as purified recombinant pol delta and its subassemblies, were analyzed. Our results indicate that p12 contributes to PCNA-dependent pol delta activity, i.e. the p12-PCNA interaction is functional. Our data indicate that both p12 and p68 are required for optimal pol delta activity. This supports the hypothesis that the interaction between pol delta and PCNA is a divalent one that involves p12 and p68. We propose a model in which pol delta interacts with PCNA via at least two of its subunits, and one in which p12 could play a role in stabilizing the overall pol delta-PCNA complex as well as pol delta itself.