习17 beta-hydroxysteroid脱氢酶类型定位人类steroidogenic细胞。

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柴Z, Brereton P,铃木T, Sasano H, Obeyesekere V,埃舍尔G, Saffery R,富勒P, Enriquez C, Krozowski Z

习17 beta-hydroxysteroid脱氢酶类型定位人类steroidogenic细胞。

内分泌学。2003年5月,144 (5):2084 - 91。

PubMed ID

12697717 (在PubMed

]

文摘

我们搜必威国际app索表达序列标签数据库与短链醇脱氢酶的保守域总科和识别的另一个同种型17 beta-hydroxysteroid脱氢酶,17βHSDXI。这种酶转换5 alpha-androstane-3α,17 beta-diol雄甾酮。衬底被卷入支持妊娠和调制γ-氨基丁酸受体的活动。与人类视网膜17βHSDXI是共线的短链脱氢酶/还原酶retSDR2,没有已知的蛋白质生物活性(加入。AAF06939)。的蛋白质与已知函数,17βHSDXI retinol-metabolizing酶retSDR1最密切相关,与它有30%的身份。有一个多态的15腺苷的cDNA序列和5 '端非翻译区C719T沉默的多态性。17βHSDXI构建一段20腺苷酸被发现产生酶活性明显多于构造包含腺苷15或更少(43%比26%,P < 0.005)。C719T多态性存在于15%的基因组DNA样本。北部污点分析显示高水平的17βHSDXI表达式在胰腺中,肾、肝、肺、肾上腺、卵巢和心脏。 Immunohistochemical staining for 17 beta HSDXI is strong in steroidogenic cells such as syncytiotrophoblasts, sebaceous gland, Leydig cells, and granulosa cells of the dominant follicle and corpus luteum. In the adrenal 17 beta HSDXI, staining colocalized with the distribution of 17 alpha-hydroxylase but was stronger in the mid to outer cortex. 17 beta HSDXI was also found in the fetus and increased after birth. Liver parenchymal cells and epithelium of the endometrium and small intestine also stained. Regulation studies in mouse Y1 cells showed that cAMP down-regulates 17 beta HSDXI enzymatic activity (40% vs. 32%, P < 0.05) and reduces gene expression to undetectable levels. All-trans-retinoic acid did not affect 17 beta HSDXI expression or activity, but addition of the retinoid together with cAMP significantly decreased activity over cAMP alone (32% vs. 23%, P < 0.05). Cloning and sequencing of the 17 beta HSDXI promoter identified the potential nuclear receptor steroidogenic factor-1 half-site TCCAAGGCCGG, and a cluster of three other potential steroidogenic factor-1 half-sites were found in the distal part of intron 1. Collectively, these results suggest a role for 17 beta HSDXI in androgen metabolism during steroidogenesis and a possible role in nonsteroidogenic tissues including paracrine modulation of 5 alpha-androstane-3 alpha, 17 beta-diol levels. 17 beta HSDXI could act by metabolizing compounds that stimulate steroid synthesis and/or by generating metabolites that inhibit it.

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药物靶点

药物	目标	类	生物	药理作用	行动
维他命A	短链脱氢酶/还原酶3	蛋白质	人类	未知的	底物	细节

多肽

的名字	UniProt ID
雌二醇17-beta-dehydrogenase 11	Q8NBQ5	细节