丙酮酸脱氢酶激活和激酶表达在人类骨骼肌在禁食。
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汤斯顿RJ Spriet LL,瓦特MJ, Mehan KA,哈格里夫斯M,卡梅伦·史密斯称D
丙酮酸脱氢酶激活和激酶表达在人类骨骼肌在禁食。
J:杂志(1985)。2004年6月,96 (6):2082 - 7。Epub 2004年2月13日。
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14966024 (在PubMed]
- 文摘
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禁食部队自适应变化的整个身体和骨骼肌代谢,增加脂肪氧化,减少碳水化合物的氧化。我们测试的假设40小时禁食会降低丙酮酸脱氢酶(PDH)活动,增加PDH激酶(此后)同种型mRNA表达人类骨骼肌。此后的假定的转录激活物同功酶、过氧物酶体proliferator-activated receptor-alpha (PPAR-alpha)蛋白质,和forkhead同族体在横纹肌肉瘤(FKHR) mRNA也测量。11名健康成人后禁食标准餐(25%的脂肪,碳水化合物60%,蛋白质15%)与血液和骨骼肌肉样本3,15日和40 h餐后。禁食增加血浆游离脂肪酸、甘油和beta-hydroxybutyrate浓度和降低血糖和胰岛素浓度。PDH活动减少从0.88 + / - 0.11更易与乙酰辅酶a。分钟(1)。公斤肌肉湿wt (1) 3 h 0.62 + / - 0.10 (P =不重要)和0.39 + / - 0.06 (P < 0.05)更易。分钟(1)。公斤湿质量(1)后15 - 40 h的禁食。 Although all four PDK isoforms were expressed in human skeletal muscle, PDK-2 and -4 mRNA were the most abundant. PDK-1 and -3 mRNA abundance was approximately 1 and 15% of the PDK-2 and -4 levels, respectively. The 40-h fast had no effect on PDK-1, -2, and -3 mRNA expression. PDK-4 mRNA was significantly increased approximately 3-fold after 15 h and approximately 14-fold after 40 h of fasting. Skeletal muscle PPAR-alpha protein and FKHR mRNA abundance were unaffected by the fast. The results suggest that decreased PDH activation after 40 h of fasting may have been a function of the large increase in PDK-4 mRNA expression and possible subsequent increase in PDK protein and activity. The changes in PDK-4 expression and PDH activity did not coincide with increases in the transcriptional activators PPAR-alpha and FKHR.