Catalase-dependent乙醇氧化灌注大鼠肝脏。通过过氧化物酶体要求fatty-acid-stimulated过氧化氢生产。

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Catalase-dependent乙醇氧化灌注大鼠肝脏。通过过氧化物酶体要求fatty-acid-stimulated过氧化氢生产。

欧元。1988年9月15日,176 (2):477 - 84。

PubMed ID

3416882 (在PubMed

]

文摘

本研究的目的是测量catalase-dependent乙醇摄入率和利率灌注大鼠肝脏过氧化氢生成的脂肪酸链长不同的的存在。的乙醇吸收从禁食大鼠肝脏,灌注在循环系统中,约80 mumol g - 1 h -减少到10级mumol g - 1 h级的乙醇脱氢酶(ADH)的抑制剂,4-methylpyrazole。medium-chain-length脂肪酸,月桂酸盐(12:0;1毫米),利率4-methylpyrazole-insensitive乙醇吸收最大限度地增加到80 - 85 mumol g - 1 h级。乙醇的吸收减少脂肪酸的碳链长度降低[hexanoate (6:0) = 23 mumol g - 1 h级;辛酸酯(译者注)= 55 mumol g - 1 h级;癸酸盐(10:0)= 65 mumol g - 1 h级)或增加[十四酸盐(14:0)= 77 mumol g - 1 h级;棕榈酸酯(16:0)= 80 mumol g - 1 h级;硬脂酸(18:0)= 29 mumol g - 1 h级;油酸(第18章)= 60 mumol g - 1 h级; erucate (22:3) = 22 mumol g-1 h-1] from 12:0. Oleate did not increase rates of hydroxylation of p-nitrophenol, a substrate for the ethanol-inducible form of cytochrome P-450, indicating that the stimulation of ethanol uptake by fatty acids was not due to increased mixed-function oxidation. The increase of ethanol uptake was also not due to displacement of 4-methylpyrazole from ADH by fatty acids, since oleate stimulated ethanol uptake by about 50% in perfused livers from deermice genetically deficient in ADH. The increase in 4-methylpyrazole-insensitive ethanol uptake by fatty acids was blocked by the catalase inhibitor, aminotriazole, indicating the involvement of catalase. Rates of H2O2 generation by livers perfused in a non-recirculating system with 1.7% albumin were increased from 6 +/- 1 to 23 +/- 5 mumol g-1 h-1 by oleate (1 mM). Because of the discrepancy between rates of ethanol metabolism and H2O2 production, methods were developed to measure H2O2 production in a recirculating perfusion system. H2O2 generation was determined from the time necessary for steady-state level of catalase-H2O2, measured spectrophotometrically (660-640 nm) through a lobe of the liver, to return to basal values after the addition of a known quantity of methanol, which is not metabolized by ADH in the rat.(ABSTRACT TRUNCATED AT 400 WORDS)

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药物靶点

药物	目标	类	生物	药理作用	行动
Fomepizole	过氧化氢酶	蛋白质	人类	是的	抑制剂	细节