小说体内CYP2A6基因表现,建立了等位基因损害体内尼古丁代谢在黑色的非洲裔人口。
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Mwenifumbo JC,艾尔Koudsi N,可,周问,霍夫曼EB,卖家EM,廷代尔射频
小说体内CYP2A6基因表现,建立了等位基因损害体内尼古丁代谢在黑色的非洲裔人口。
哼Mutat。2008; 29 (5): 679 - 88。doi: 10.1002 / humu.20698。
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18360915 (在PubMed]
- 文摘
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细胞色素P450 2 a6(体内CYP2A6基因表现)是一种人类最出名的酶代谢与烟草相关的化合物,如尼古丁,可替宁(COT)和亚硝胺前致癌物。体内CYP2A6基因表现的遗传变异与吸烟有关状态,香烟消费,与烟草有关的癌症。我们目标是功能描述四个体内CYP2A6基因表现产生的序列变异对他们的单体型,等位基因频率,并与体内体内CYP2A6基因表现的活动。体内,尼古丁是口服281名志愿者的非洲黑人后裔。血液样本收集体内CYP2A6基因表现为动能表现型和基因型。体外,尼古丁C-oxidation不等的表达变异酶的催化效率进行评估。四个无特征序列变异被发现在七体内CYP2A6基因表现小说的等位基因(*)24方式;(*)(*)25日26日(*)27日和28日方式,大多数人体内CYP2A6基因表现与体内受损相关活动。基于体内的尼古丁代谢组,数据变异的等位基因,。意味着trans-3 -hydroxycotinine /可替宁(3 hc /床)不同(P < 0.001)之间的正常(100%)、中级(64%)和慢速(40%)组。 Systemic exposure to nicotine following oral administration also differed (P<0.001) between normal (100%), intermediate (139%), and slow (162%) metabolism groups. In addition, alleles of individuals with unusual phenotype-genotype relationships were sequenced, resulting in the discovery of five novel uncharacterized alleles and at least one novel duplication allele. A total of 7% of this population of Black African descent had at least one of the eight novel characterized alleles and 29% had at least one previously established allele. These findings are important for increasing the accuracy of association studies between CYP2A6 genotype and behavioral, disease, or pharmacological phenotypes.