蛋氨酸再生和转氨酶在枯草芽孢杆菌、蜡样芽胞杆菌、炭疽杆菌。

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伯杰BJ,英语年代,陈G, Knodel MH

蛋氨酸再生和转氨酶在枯草芽孢杆菌、蜡样芽胞杆菌、炭疽杆菌。

J Bacteriol。2003年4月,185(8):2418 - 31所示。

PubMed ID

12670965 (在PubMed

]

文摘

ketomethiobutyrate蛋氨酸一直以前检查的转换在许多生物体,其中转氨酶负责的反应被发现是成员Ia亚科(l·c·伯杰j .威尔逊·伍德b·j·伯杰,j . Bacteriol。183:4421 - 4434, 2001)。枯草芽孢杆菌的基因组被发现不包含亚Ia转氨酶序列。相反,类似酶的枯草芽孢杆菌被发现如果亚科的成员。这些假定的天冬氨酸转氨酶,yugH、ywfG ykrV, aspB, patA基因产物,已经被克隆,表达,蛋氨酸再生活动的特征。只有YkrV ketomethiobutyrate转换为蛋氨酸,只有当谷氨酰胺,它催化反应是用作氨基供体。相比之下,亚细胞匀浆的枯草芽孢杆菌和蜡样芽胞杆菌利用亮氨酸,异亮氨酸,缬氨酸,丙氨酸,苯丙氨酸和酪氨酸氨基捐赠者同样有效。这两个假定的支链氨基转移酶基因在枯草芽孢杆菌,ybgE ywaA,也被克隆、表达和特征。两个基因产物有效促转氨支链氨基酸和酮戊二酸,但只有YbgE转换ketomethiobutyrate蛋氨酸。氨基供体偏爱蛋氨酸再生YbgE被发现亮氨酸、异亮氨酸、缬氨酸、苯丙氨酸和酪氨酸。枯草芽孢杆菌的ybgE基因家族的成员三世转氨酶和瀑布的一个亚科指定III a。 Examination of B. cereus and Bacillus anthracis genome data found that there were no subfamily IIIa homologues in these organisms. In both B. cereus and B. anthracis, two putative branched-chain aminotransferases and two putative D-amino acid aminotransferases were discovered as members of subfamily IIIb. These four sequences were cloned from B. cereus, expressed, and characterized. Only the gene product from the sequence designated Bc-BCAT2 was found to convert ketomethiobutyrate to methionine, with an amino donor preference of leucine, isoleucine, valine, phenylalanine, and tyrosine. The B. anthracis homologue of Bc-BCAT2 was also cloned, expressed, and characterized and was found to be identical in activity. The aminooxy compound canaline was found to be an uncompetitive inhibitor of B. subtilis YbgE and also inhibited growth of B. subtilis and B. cereus in culture.

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药物靶点

药物	目标	类	生物	药理作用	行动
异亮氨酸	Branched-chain-amino-acid转氨酶,线粒体	蛋白质	人类	未知的	不可用	细节
亮氨酸	Branched-chain-amino-acid转氨酶,线粒体	蛋白质	人类	未知的	不可用	细节